A mechanism based protein crosslinker for acyl carrier protein dehydratases
نویسندگان
چکیده
منابع مشابه
Versatility of acyl-acyl carrier protein synthetases.
The acyl carrier protein (ACP) requires posttranslational modification with a 4'-phosphopantetheine arm for activity, and this thiol-terminated modification carries cargo between enzymes in ACP-dependent metabolic pathways. We show that acyl-ACP synthetases (AasSs) from different organisms are able to load even, odd, and unnatural fatty acids onto E. coli ACP in vitro. Vibrio harveyi AasS not o...
متن کاملAcyl carrier protein. XVII. Purification and properties of -hydroxyacyl acyl carrier protein dehydrase.
/3-Hydroxyacyl acyl carrier protein (ACP) dehydrase has been purified 2900-fold from extracts of Escherichia coli. The enzyme catalyzes the reversible dehydration of /3-hydroxyacyl-ACP thioesters to yield specifically tram-2-enoylACP products. It is active with frans-2-enoyl-ACP thioesters of chain lengths from 4 through 16 carbon atoms. The enzyme catalyzes the hydration of cis-5frans-2-dodeca...
متن کاملP-Ketoacyl-Acyl Carrier Protein Synthetase
/3-Ketoacyl-acyl carrier protein (ACP) synthetase catalyzes the condensation reaction of fatty acid synthesis in Escherichia coli. The homogeneous enzyme reacts with hexanoylCoA to form hexanoyl-enzyme which was isolated and characterized. Hexanoyl-enzyme contains 2 mol of hexanoate/mol of enzyme (molecular weight 66,000); it is labile at alkaline pH, and it reacts with neutral hydroxylamine to...
متن کاملPlant holo-(acyl carrier protein) synthase.
1. An improved method was developed for the assay of plant holo-(acyl carrier protein) synthase activity, using Escherichia coli acyl-(acyl carrier protein) synthetase as a coupling enzyme. 2. Holo-(acyl carrier protein) synthase was partially purified from spinach (Spinacia oleracea) leaves by a combination of (NH4)2SO4 fractionation and anion-exchange and gel-permeation chromatography. 3. The...
متن کاملEnzymatic desaturation of stearyl acyl carrier protein.
Stearyl acyl carrier protein, chemically prepared from 1-W stearic acid and Escherichia co2i acyl carrier protein, is desaturated to oleic acid by extracts of photoauxotrophic Euglena gracilis and of spinach. The soluble stearyl acyl carrier protein desaturase system from Eug~ena has been separated into three components, a reduced triphosphopyridine nucleotide oxidase, the desaturase, and a non...
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ژورنال
عنوان ژورنال: Bioorganic & Medicinal Chemistry Letters
سال: 2010
ISSN: 0960-894X
DOI: 10.1016/j.bmcl.2010.06.028